Ground sections are ultra-thin slices of hard tissues such as teeth and bones that are meticulously prepared to study their detailed internal structure under a microscope. These sections are essential in various fields like histology (the study of tissue architecture), forensic odontology (identifying individuals based on dental evidence), and dental education (teaching students the microscopic anatomy of teeth).Tooth ground sectioning is considered an invaluable technique for studying dental anatomy, especially for visualizing the relationship between enamel, dentin, pulp chamber, and surrounding cementum. It has the added advantage of being a non-chemical and non-destructive method, thus preserving the tooth's natural architecture. For instance, in educational settings, ground sections help students observe the intricate patterns of enamel prisms or sclerotic dentin in aging teeth.This book aims to comprehensively explore the apparatus required, the techniques employed , and the recent advancements in the preparation and analysis of tooth ground sections.