The venom of wasps and spiders has been considered an important source ofneuroactive compounds. However, several molecules with neuropharmacological potential still remain unknown, mainly due to the scarcity of studies focusing the scarcity of studies focusing on neuroactive compounds present in wasps and spiders venom of Neotropical wasps and spiders. In the Central Nervous System (CNS), these molecules can act on different neuronal targets (receptors targets (receptors, transporters and ion channels) and have been used as neurological diseases, as well as for the development of new development of new model drugs in the treatment of epilepsy and chronic pain. Thus, the objectives of this study were to evaluate the the neurobiological activity of the crude and denatured venom of the social wasp Polybia occidentalis, as well as to identify and characterize the compounds that presented anticonvulsant and antinociceptive effects when injected into the in the CNS of rats.