This second edition provides new and updated methods that detail new DNA nanotechnology techniques. Chapters focus on DNA origami nanostructures for arranging matter in the nanoscale or on their manipulation with the aid of other technologies, on procedures for making nucleic acids nanostructures of different kinds, and methods to simulate complex nanostructures or to use them in biosensing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, DNA Nanotechnology: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

DNA Nanotechnology: From the Pub to Information-based Chemistry.- The Synthesis of Designer DNA.- Synthesis of DNA-based Nanowires.- Preparation of Anti-miR PNAs for Drug Development and Nanomedicine.- Loading of PNA and other Molecular Payloads on Inorganic Nanostructures for Theranostics.- Design and Characterization of pH-triggered DNA Nanoswitches and Nanodevices Based on DNA Triplex Structures.- Characterizing Surface-immobilized DNA Structures and Devices Using a Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D).- Hybridization Chain Reaction Design and Biosensor Implementation.- Ultrasensitive Detection of Metal Ions with DNA Nanostructure.- Spatially Resolved Peptide-DNA Nanoassemblages for Biomarker Detection; A Synergy of DNA Directed Immobilization and Nanografting.- LSPR Detection of Nucleic Acids on Nanoparticle Monolayers.- DNA-templated Silver Nanoclusters for DNA Methylation Detection.- Selection of Structure-switching DNA Aptamers Binding SolubleSmall Molecules and SPR Validation of Enrichment Dielectrophoretic Stretching of DNA.- A Practical Guide to Molecular Dynamics Simulations of DNA Origami Systems.- Single-molecule Patterning via DNA Nanostructure Assembly: A Reusable Platform.- Directed Protein Adsorption through DNA Origami Masks.- DNA Origami Structures Interfaced to Inorganic Nanodevices.- Tuning Gold Nanoparticles Plasmonic Properties by DNA Nanotechnology.- DNA-assisted Molecular Lithography.- Constructing Free Energy Landscapes of Nucleic Acid Hairpin Unfolding.